[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].
Identifieur interne : 005C72 ( Main/Exploration ); précédent : 005C71; suivant : 005C73[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].
Auteurs : Xin-Yu Tan [République populaire de Chine] ; Zheng Fan ; Hua-Jin Wang ; Lei Shi ; Bin Yin ; An-Ping Ni ; Chuan Qin ; Ke Zou ; Yan Shen ; Jian-Gang Yuan ; Bo-Qin Qiang ; Xiao-Zhong PengSource :
- Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae [ 1000-503X ] ; 2003.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN viral (génétique), ARN viral (génétique), Analyse de séquence d'ADN, Clonage moléculaire, Données de séquences moléculaires, Escherichia coli (génétique), Génome viral, Protéines de fusion recombinantes (biosynthèse), Protéines de fusion recombinantes (génétique), Protéines nucléocapside (biosynthèse), Protéines nucléocapside (génétique), Protéines nucléocapside (isolement et purification), RT-PCR, Séquence d'acides aminés, Séquence nucléotidique, Vecteurs génétiques, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- biosynthèse : Protéines de fusion recombinantes, Protéines nucléocapside.
- génétique : ADN complémentaire, ADN viral, ARN viral, Escherichia coli, Protéines de fusion recombinantes, Protéines nucléocapside, Virus du SRAS.
- isolement et purification : Protéines nucléocapside, Virus du SRAS.
- Analyse de séquence d'ADN, Clonage moléculaire, Données de séquences moléculaires, Génome viral, RT-PCR, Séquence d'acides aminés, Séquence nucléotidique, Vecteurs génétiques.
English descriptors
- KwdEn :
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, DNA, Complementary (genetics), DNA, Viral (genetics), Escherichia coli (genetics), Genetic Vectors, Genome, Viral, Molecular Sequence Data, Nucleocapsid Proteins (biosynthesis), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (isolation & purification), RNA, Viral (genetics), Recombinant Fusion Proteins (biosynthesis), Recombinant Fusion Proteins (genetics), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), Sequence Analysis, DNA.
- MESH :
- chemical , biosynthesis : Nucleocapsid Proteins, Recombinant Fusion Proteins.
- chemical , genetics : DNA, Complementary, DNA, Viral, Nucleocapsid Proteins, RNA, Viral, Recombinant Fusion Proteins.
- genetics : Escherichia coli, SARS Virus.
- chemical , isolation & purification : Nucleocapsid Proteins, SARS Virus.
- Amino Acid Sequence, Base Sequence, Cloning, Molecular, Genetic Vectors, Genome, Viral, Molecular Sequence Data, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis, DNA.
Abstract
To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.
PubMed: 14650145
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003075
- to stream PubMed, to step Curation: 003075
- to stream PubMed, to step Checkpoint: 002F56
- to stream Ncbi, to step Merge: 000474
- to stream Ncbi, to step Curation: 000474
- to stream Ncbi, to step Checkpoint: 000474
- to stream Main, to step Merge: 006142
- to stream Main, to step Curation: 005C72
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].</title><author><name sortKey="Tan, Xin Yu" sort="Tan, Xin Yu" uniqKey="Tan X" first="Xin-Yu" last="Tan">Xin-Yu Tan</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 10005, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 10005</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Fan, Zheng" sort="Fan, Zheng" uniqKey="Fan Z" first="Zheng" last="Fan">Zheng Fan</name></author><author><name sortKey="Wang, Hua Jin" sort="Wang, Hua Jin" uniqKey="Wang H" first="Hua-Jin" last="Wang">Hua-Jin Wang</name></author><author><name sortKey="Shi, Lei" sort="Shi, Lei" uniqKey="Shi L" first="Lei" last="Shi">Lei Shi</name></author><author><name sortKey="Yin, Bin" sort="Yin, Bin" uniqKey="Yin B" first="Bin" last="Yin">Bin Yin</name></author><author><name sortKey="Ni, An Ping" sort="Ni, An Ping" uniqKey="Ni A" first="An-Ping" last="Ni">An-Ping Ni</name></author><author><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name></author><author><name sortKey="Zou, Ke" sort="Zou, Ke" uniqKey="Zou K" first="Ke" last="Zou">Ke Zou</name></author><author><name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name></author><author><name sortKey="Yuan, Jian Gang" sort="Yuan, Jian Gang" uniqKey="Yuan J" first="Jian-Gang" last="Yuan">Jian-Gang Yuan</name></author><author><name sortKey="Qiang, Bo Qin" sort="Qiang, Bo Qin" uniqKey="Qiang B" first="Bo-Qin" last="Qiang">Bo-Qin Qiang</name></author><author><name sortKey="Peng, Xiao Zhong" sort="Peng, Xiao Zhong" uniqKey="Peng X" first="Xiao-Zhong" last="Peng">Xiao-Zhong Peng</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:14650145</idno><idno type="pmid">14650145</idno><idno type="wicri:Area/PubMed/Corpus">003075</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003075</idno><idno type="wicri:Area/PubMed/Curation">003075</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003075</idno><idno type="wicri:Area/PubMed/Checkpoint">002F56</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002F56</idno><idno type="wicri:Area/Ncbi/Merge">000474</idno><idno type="wicri:Area/Ncbi/Curation">000474</idno><idno type="wicri:Area/Ncbi/Checkpoint">000474</idno><idno type="wicri:doubleKey">1000-503X:2003:Tan X:cloning:expression:and</idno><idno type="wicri:Area/Main/Merge">006142</idno><idno type="wicri:Area/Main/Curation">005C72</idno><idno type="wicri:Area/Main/Exploration">005C72</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].</title><author><name sortKey="Tan, Xin Yu" sort="Tan, Xin Yu" uniqKey="Tan X" first="Xin-Yu" last="Tan">Xin-Yu Tan</name><affiliation wicri:level="1"><nlm:affiliation>National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 10005, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>National Laboratory of Medical Molecular Biology, Institute of Basic Medical Sciences, CAMS and PUMC, Beijing 10005</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Fan, Zheng" sort="Fan, Zheng" uniqKey="Fan Z" first="Zheng" last="Fan">Zheng Fan</name></author><author><name sortKey="Wang, Hua Jin" sort="Wang, Hua Jin" uniqKey="Wang H" first="Hua-Jin" last="Wang">Hua-Jin Wang</name></author><author><name sortKey="Shi, Lei" sort="Shi, Lei" uniqKey="Shi L" first="Lei" last="Shi">Lei Shi</name></author><author><name sortKey="Yin, Bin" sort="Yin, Bin" uniqKey="Yin B" first="Bin" last="Yin">Bin Yin</name></author><author><name sortKey="Ni, An Ping" sort="Ni, An Ping" uniqKey="Ni A" first="An-Ping" last="Ni">An-Ping Ni</name></author><author><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name></author><author><name sortKey="Zou, Ke" sort="Zou, Ke" uniqKey="Zou K" first="Ke" last="Zou">Ke Zou</name></author><author><name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name></author><author><name sortKey="Yuan, Jian Gang" sort="Yuan, Jian Gang" uniqKey="Yuan J" first="Jian-Gang" last="Yuan">Jian-Gang Yuan</name></author><author><name sortKey="Qiang, Bo Qin" sort="Qiang, Bo Qin" uniqKey="Qiang B" first="Bo-Qin" last="Qiang">Bo-Qin Qiang</name></author><author><name sortKey="Peng, Xiao Zhong" sort="Peng, Xiao Zhong" uniqKey="Peng X" first="Xiao-Zhong" last="Peng">Xiao-Zhong Peng</name></author></analytic><series><title level="j">Zhongguo yi xue ke xue yuan xue bao. Acta Academiae Medicinae Sinicae</title><idno type="ISSN">1000-503X</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>DNA, Complementary (genetics)</term><term>DNA, Viral (genetics)</term><term>Escherichia coli (genetics)</term><term>Genetic Vectors</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Nucleocapsid Proteins (biosynthesis)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (isolation & purification)</term><term>RNA, Viral (genetics)</term><term>Recombinant Fusion Proteins (biosynthesis)</term><term>Recombinant Fusion Proteins (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN viral (génétique)</term><term>ARN viral (génétique)</term><term>Analyse de séquence d'ADN</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Escherichia coli (génétique)</term><term>Génome viral</term><term>Protéines de fusion recombinantes (biosynthèse)</term><term>Protéines de fusion recombinantes (génétique)</term><term>Protéines nucléocapside (biosynthèse)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (isolement et purification)</term><term>RT-PCR</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Vecteurs génétiques</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Complementary</term><term>DNA, Viral</term><term>Nucleocapsid Proteins</term><term>RNA, Viral</term><term>Recombinant Fusion Proteins</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines de fusion recombinantes</term><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN complémentaire</term><term>ADN viral</term><term>ARN viral</term><term>Escherichia coli</term><term>Protéines de fusion recombinantes</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Cloning, Molecular</term><term>Genetic Vectors</term><term>Genome, Viral</term><term>Molecular Sequence Data</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Clonage moléculaire</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>RT-PCR</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To clone, express and purify nucleocapsid protein from SARS coronavirus PUMC2 strain.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Fan, Zheng" sort="Fan, Zheng" uniqKey="Fan Z" first="Zheng" last="Fan">Zheng Fan</name><name sortKey="Ni, An Ping" sort="Ni, An Ping" uniqKey="Ni A" first="An-Ping" last="Ni">An-Ping Ni</name><name sortKey="Peng, Xiao Zhong" sort="Peng, Xiao Zhong" uniqKey="Peng X" first="Xiao-Zhong" last="Peng">Xiao-Zhong Peng</name><name sortKey="Qiang, Bo Qin" sort="Qiang, Bo Qin" uniqKey="Qiang B" first="Bo-Qin" last="Qiang">Bo-Qin Qiang</name><name sortKey="Qin, Chuan" sort="Qin, Chuan" uniqKey="Qin C" first="Chuan" last="Qin">Chuan Qin</name><name sortKey="Shen, Yan" sort="Shen, Yan" uniqKey="Shen Y" first="Yan" last="Shen">Yan Shen</name><name sortKey="Shi, Lei" sort="Shi, Lei" uniqKey="Shi L" first="Lei" last="Shi">Lei Shi</name><name sortKey="Wang, Hua Jin" sort="Wang, Hua Jin" uniqKey="Wang H" first="Hua-Jin" last="Wang">Hua-Jin Wang</name><name sortKey="Yin, Bin" sort="Yin, Bin" uniqKey="Yin B" first="Bin" last="Yin">Bin Yin</name><name sortKey="Yuan, Jian Gang" sort="Yuan, Jian Gang" uniqKey="Yuan J" first="Jian-Gang" last="Yuan">Jian-Gang Yuan</name><name sortKey="Zou, Ke" sort="Zou, Ke" uniqKey="Zou K" first="Ke" last="Zou">Ke Zou</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Tan, Xin Yu" sort="Tan, Xin Yu" uniqKey="Tan X" first="Xin-Yu" last="Tan">Xin-Yu Tan</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 005C72 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 005C72 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:14650145
|texte= [Cloning, expression and purification of SARS coronavirus PUMC2 strain nucleocapsid protein].
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:14650145" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |